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"Nightshade" may refer to:
In-universe
- Nightshade, any of several science fiction serials from early BBC television. (PROSE: Nightshade, HOMEVID: The Zero Imperative)
- Professor Nightshade, the title character portrayed by Edmund Trevithick
- Nightshade: The Motion Picture (COMIC: Star Beast II)
- The plant deadly nightshade, otherwise known as Atropa belladonna.
Behind the scenes
- A Doctor Who Virgin New Adventures novel titled Nightshade, written by Mark Gatiss and featuring the Seventh Doctor and Ace.
- The audio dramatisation of the novel.