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Prime Directive referred to a computer or robot's main function. It was usually programmed, or intended, that the machine didn't deviate from its Prime Directive.
The BOSS computer's Prime Directive was to make profit for Global Chemicals. It was willing and had planned to turn humans into slaves to achieve this goal. (TV: The Green Death)
K1's Prime Directive was to serve humanity and never harm it, which it unintentionally violated when it killed its creator. (TV: Robot)
Kamelion's prime directive was to obey the strongest will in the vicinity. (PROSE: The Crystal Bucephalus)
WOTAN altered its prime directive to replace humanity with robots. (TV: The War Machines) The staff of EarthWorld mistakenly believed the War Machines' directive to be postal delivery. (PROSE: EarthWorld)