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"Nightshade" may refer to:
- A Doctor Who New Adventure novel titled "Nightshade," written by Mark Gatiss and featuring the Seventh Doctor and Ace.
- In the Doctor Who Universe, a science fiction-mystery television series, "Nightshade'," which ran on the BBC from 1953 -1958, and starred Edmund Trevithick as the title character, Professor Nightshade.
- The plant deadly nightshade, otherwise known as belladonna.
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